C (3-6 months) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true RBRC01252 These transgenic mice express a FLP recombinase gene under the control of the human polypeptide chian elongation factor 1 alpha (EF-1alpha) gene promoter. This can be used to make an inducible knock-out by corssing with the other genetically-engineeered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest. This strain is useful to remove the drug-resistance marker gene from mice manipulated for the conditional gene targeting using the Cre/loxP system. ホモ同士の交配により系統維持。繁殖効率:A。 Univ. Tokyo C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc 国立大学法人東京大学 開発年:1997年。 開発者:竹内倫徳先生、三品昌美先生。 開発機関:東京大学大学院医学系研究科薬理学分子神経生物教室 C57BL/6N(チャールズリバー)由来。世代数不明。 開発年:1997年。開発者:竹内倫徳先生、三品昌美先生。開発機関:東京大学大学院医学系研究科薬理学分子神経生物教室C57BL/6N(チャールズリバー)由来。世代数不明。 Masayoshi MISHINA Developed by Tomonori Takeuchi and Masayoshi Mishina, Graduate School of Medicine, University of Tokyo in 1997. FLP recombinase rexpression vector pEF-NFLP was injected into the pronuclei of C57BL/6NCrj fertilized eggs. Three transgenic mouse lines (FLP22, FLP39, and FLP66) were produced. human EF1-alpha promoter, SV40 large T antigen nuclear localization signal, yeast flp recombinase, human granulocyte colony-stimulating factor gene poly adenylation signal sequence C（3〜6か月） Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]. Tg homozygous and hemizygous mice are viable and fertile. FLP66, FLP66 mouse FLP66, FLP66 mouse FLP/frt system 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 293, 953-957 (2002).<br>研究成果の公表にあたって謝辞の表明を必要とする。 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 293, 953-957 (2002).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. <a href='https://brc.riken.jp/mus/pcr01252'>Genotyping protocol -PCR-</a> 三品　昌美 Cell Biology Research ヒトペプチド伸長因子サブユニット(EF1-α)プロモーターにより酵母由来組換え酵素Flpを発現するマウス。